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离体玉米精细胞的电泳分析与分离
其生物学研究进展．植物学集刊，I993，7：3483粱子钧，施永德．细胞电泳技术及其在生物学和医学中的应月．生物化学与生物物理进展，1976，8(1)：544千目成，秦鹏春．x,[link widoczny dla zalogowanych]，Y精子分离技术的进展．生物学杂志，1989,4：35I-IalimH，PearceRS．AneteO．roph~reticmethodforbulkmampulationofisolatedprotoplastsm崦plants．BiochemP~iolPfhr∞L1980,[link widoczny dla zalogowanych]。175：123。6Koon~aF．JacobsenHJ．El~'tmphoret．ics~a／"dlionandcbaTa0nofpeaprotoplasts．Phy~io]ogiaPlantanum1．I992．85：2847严志坚，唐佩华．玉米精细胞的大量分离及其细胞电泳分析．见：中国植物学会．中国植物学会60周年学术报告及论文摘要编北京：中国科学技术出版社，I993．3308G她LR，CutlerAJ．Slr,[link widoczny dla zalogowanych],ir~lPD，etalIsoelectricfoeus~ofplantcellprotoplastsseparationofdjmprotoplasttypesPlantPh-：~iol,1985,77：7659陈季楚，傅婉体．植物原生质膜表面电荷性质的研究I：pn及某些酶制剂对元麦叶肉原生质体电泳率的影响实验生物学报，1980．13：28111{匮Cy)I砌lREⅡCANAIYSlSAND口0CTR0)】UⅡlcSEPARAT【0N0lFSP口暖MCⅡIS硅；0IAT口DFR0MZMAL．JHuawu)TangPel~lla)(1)DepartmentofBiology，BeijingNormalUniversity，100875．Beijing,[link widoczny dla zalogowanych]，PRC：2)InstJtuteofBotanyAcademia$inica，100044．Beijing，PRC)Abslraet1rheelectrophoreticmobility饵M)ofspermcells(sIisolatedfromZeama．1'sLwasmeasuredandappearedthatthepHofelectrophoreticmediumeouldeffectgreaⅡyonEM．11leisoelectricpointofSCinthisstudywasbetween2．4and3．0．InMES(DH6．71buffer．statisticanalys~sofresultsofSCEMdemom'tmtedthattherr1a$ofSCmightbedi-videdintotwogroupsbytheirdiscrepantEMwhichwere1．803xlOand1．667x10m·V·s—respectively．Accordi,lgtothedifferencesofSCEM．山ecalTi~一freeelectrophoresisusedtoseparateSCintotwomaingroupswhichhad2nmadistanceofEMapartfromeachother．11heviabilityofSCafterel~rophoresiseouldmaintainhighleve1．Keywordscytophoreticanatysis,[link widoczny dla zalogowanych]；SpelTI1heteromophism；caiiier—freeelectrophoresis；Zeank,7~,sL
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