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Diagnosis and treatment of guinea fowl outbreak of Newcastle disease


01) 【Key Words 1s858.31 IB 【【Document Code Article ID 11672-2078 (2008) 01-0037-01 guinea fowl, also known as guinea fowl, pearl birds, Guinea birds. Guinea fowl is rich in amino acids the body needs, protein, and contains a high mineral and trace elements contained in the vitamin than 1 times higher than chicken. For disease resistance and high survival rate, ease of breeding farm, with high economic value. An incidence of a chicken households Shulan City, in April 2007 from the Jilin left home to buy a special 3 day-old poultry farm were caged guinea fowl 300, l0 days old drinking immune Newcastle disease attenuated vaccine, l5 days of age incidence of some guinea fowl, 3d disease after 146, which killed 46. 2 In addition to acute symptoms of clinical diagnosis of death no clinical symptoms of guinea fowl, guinea fowl most of the remaining patients showed temperature 42 ~ 43 ℃, loss of appetite or waste must, mental Weidun, do not want to walk, eyes half closed or fully closed, the side or both sides of the wing down, was sleeping like. Serious illness guinea fowl breathing difficulties, nasal mucus fluid, shaking their heads or do not swallow, put down when the yellow comes out from the mouth of mucus. Yellowish green or yellow stool white Xifen. Also showed a slightly longer duration of neck to back, side or both sides of the wing can not restore the sagging, limp, standing firm, individual walking around in circles or to the online impact. The general course of 1 ~ 4d. A total of 3 pathological autopsy dissection died only l2 guinea fowl, see how the amount of yellow mucus mouth, crop full of sour liquid; legs, chest muscles with severe spotting; the junction of the esophagus and the glandular stomach and the proventriculus junction with the muscle of the stomach mucosal edema, hemorrhage, or bleeding spots strip, ulcers, glandular stomach bleeding in varying degrees; muscular stomach bleeding spots under the cuticle; intestinal bleeding in varying degrees, cecal tonsil enlargement , hemorrhage, necrosis ulcers; cloacal mucosa of the fallopian tubes and severe bleeding. 4 Laboratory of Bacteriology and Parasitology Diagnostic Check 4.1 to take dead pearl liver, spleen do contact sheets, staining, and no small Received: November 2, 2007 Tel ,0431 -87989877 / 86814288, 86814188E-mail: zhubian @ sina1O0. com bacteria; the liver and spleen tissue of blood inoculated in MacConkey and nutrient agar, 37 * (2 cultured 48h, no bacterial growth. scraping dead pearl Intestinal mucosa and contents of the microscopic examination of the body was not found parasite eggs and . 4.2 to take dead virus isolation and identification of sterile guinea fowl spleen and brain tissue emulsion made of 1:10, and added per milliliter of penicillin 2000IU,[link widoczny dla zalogowanych], streptomycin 2000lag, set at 4 ℃ for 3h, then 4000r/min centrifugal 15min,[link widoczny dla zalogowanych], respectively, the supernatant 0.2mL, inoculated with l0 day old SPF chick embryo chorioallantoic cavity, collected after 72h culture allantoic fluid and amniotic fluid, respectively, the standard positive serum with Newcastle disease, IBD standard positive serum,[link widoczny dla zalogowanych], Avian influenza made positive serum hemagglutination (HA) and hemagglutination inhibition test (H1). Results allantoic fluid and amniotic fluid of chicken to make 1% of the standard of health, the direct agglutination titer of red blood cells were 1:80 and 1:160; fixed dilution of positive serum hemagglutination inhibition allantoic fluid test (HI), the results of inhibition of Newcastle disease virus price is positive serum 1:40; and saline, Bursa, no bird flu positive serum hemagglutination inhibition price. by clinical symptoms, pathological changes and laboratory diagnosis can be confirmed as the outbreak of Newcastle disease, whereby the development of rational control measures. 5 5.1 antibody injection control measures and vaccination of chicken chickens with and without the rapid onset of segregation, and transfer chicken coops, IBD immediately bivalent Newcastle disease and emergency intramuscular yolk antibody: incidence of chicken each 0.5mL, not the incidence of chicken each 0.2mL, 3d repeated injection interval, a total of 3 injections, during the dry yeast with fluorine piperazine acid and vitamin additives,[link widoczny dla zalogowanych], spices control secondary infection. 7d after treatment of chickens with all the pearls LaSota Newcastle disease attenuated vaccine subcutaneous injection of oil emulsion, each 0.2mL. 10d recovered after the chickens, no new disease chickens,[link widoczny dla zalogowanych], the epidemic under control. 5.2 Disinfection will sick burn dead chickens, faeces buried; poultry house and apparatus poisoned with 2% solution per spray washing, immersion; closed doors, windows and sheds fumigation with formaldehyde vapor, with the same amount of ammonium hydroxide for 1 week before and in use. Head 37 *
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