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Porcine oviduct epithelial cells, granule cells, ear epithelial cells and fetal fibroblast cells in culture


Fast-dimensional cell growth, density 14 × 1o '/ ml, generally two days to generation. 4 cells compared to granulosa cells of large size, the slowest growth rate, passage density to maintain 2 × lO ~ / ml or more appropriate. After digestion, single cells and together with the remaining cell mass culture, fast growth,[link widoczny dla zalogowanych], which Wangli Hu (20o1) results. 2.3 ear epithelial cells and fetal fibroblast cells in primary culture of purified adherent monolayer cultures of cells or cell mass after digestion and passage by the confluence of the growth of adherent cells in monolayer plus trypsin, 37 ℃ 7 minutes to digest so,[link widoczny dla zalogowanych], fibroblasts gradually rounded off the wall, with the pipette tips gently blow, fibroblast-like cells can be suspended, siphoning off digestion. The original culture dish then add trypsin, 37 ℃ for 5 minutes or so to digest, wind and percussion cells were collected, then the cells were collected mainly epithelioid cells. And then inoculated culture was discarded when the next passage 4-5 minutes before the digestion of cell suspension,[link widoczny dla zalogowanych], combined with trypsin digestion,[link widoczny dla zalogowanych], cells were collected by 2-5 on behalf of the choices available purified ear epithelial cells. This result is Li Yuqiang, et al (2000) J goat skin cells similar to the results. Purification of fetal fibroblast cells and purified similar to the ear. Digest 57 minutes before collecting the cells,[link widoczny dla zalogowanych], then inoculated with culture, when the next passage and then take 4-5 minutes before the cells were 24-generation selection, get purified fetal fibroblasts. Kouquan An, et al (1999) and repeated use of enzyme digestion adherence, after 2-3 generations, get purified rabbit fibroblasts.
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